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. 2017 Jun 26;8:687. doi: 10.3389/fimmu.2017.00687

Figure 4.

Figure 4

Subtype specificity of antibodies elicited by F1mutV-protective antigen (PA) triple antigen in mice. The panels show F1V-specific IgG1 (TH1) (A) and IgG2a (TH2) (B) titers and PA-specific IgG1 (C) and IgG2a (D) titers. Mice were immunized (intramuscular) according to Figures 3A,B. Sera were collected according to Figure 3B and analyzed by ELISA. “*”, “**”, and “***” denote p < 0.05, p < 0.01, and p < 0.001, respectively (analysis of variance).