Table 1.
Xanthomonas strains and plasmids used in this study.
| Bacterial strains and plasmids | Description | Reference |
|---|---|---|
| pVO155 | pUC119-derived suicide vector (KmR) | Oke and Long, 1999 |
| pVO155::hrcT | pVO155 containing a 419-bp internal hrcT fragment from CFBP 2054 | This study |
| pVO155::hpaT | pVO155 containing a 410-bp internal hpaT fragment from CFBP 2054 | This study |
| pVO155::hgiB | pVO155 containing a 430-bp internal hgiB fragment from CFBP 2054 | This study |
| pBBR1MCS-5 | Broad-host-range vector (GmR) | Kovach et al., 1995 |
| pHrcT | pBBR1MCS-5 containing hrcT from CFBP 2541 | This study |
| pHpaT | pBBR1MCS-5 containing hpaT from CFBP 2541 | This study |
| pHpaH | pBBR1MCS-5 containing hpaH from CFBP 2541 | This study |
| pLHrpF | pLAFR3 containing the hrpF gene from X. euvesicatoria strain 85-10 | Büttner et al., 2002 |
| pBBR1MCS-5::hrpG∗ | pBBR1MCS-5 containing the hrpG∗ allele from X. euvesicatoria strain 85-10, which renders hrp gene expression constitutive in rich medium | Jacobs et al., 2015 |
| pBBR1MCS-5::hrpX | pBBR1MCS-5 containing the hrpX gene from X. euvesicatoria strain 85-10 | Koebnik et al., 2006 |
| CFBP 2054R | X. translucens pv. translucens (RfR) | This study |
| CFBP 2541R | X. translucens pv. cerealis (RfR) | Pesce et al., 2015b |
| UPB787R | X. translucens pv. translucens (RfR) | This study |
| UPB820R | X. translucens pv. hordei (RfR) | This study |
| UPB820R (pBBR1MCS-5) | UPB820R containing the empty vector pBBR1MCS-5 | This study |
| UPB820R (pBBR1MCS-5::hrpG∗) | UPB820R containing pBBR1MCS-5::hrpG∗ | This study |
| UPB820R (pBBR1MCS-5::hrpX) | UPB820R containing pBBR1MCS-5::hrpX | This study |
| UPB787R hrcT- | UPB787R with pVO155 inserted into hrcT | This study |
| UPB820R hrcT- | UPB820R with pVO155 inserted into hrcT | This study |
| UPB787R hpaT- | UPB787R with pVO155 inserted into hpaT | This study |
| UPB820R hpaT- | UPB820R with pVO155 inserted into hpaT | This study |
| UPB787R hgiB- | UPB787R with pVO155 inserted into hgiB | This study |
| UPB820R hgiB- | UPB820R with pVO155 inserted into hgiB | This study |
| UPB787R hrcT- (ev) | UPB787R hrcT- with pBBR1MCS-5 (empty vector) | This study |
| UPB787R hrcT- (pHrcT) | UPB787R hrcT- complemented with pHrcT | This study |
| UPB787R hpaT- (pHpaT) | UPB787R hpaT- complemented with pHpaT | This study |
| UPB787R hpaT- (pHpaH) | UPB787R hpaT- complemented with pHpaH | This study |
| UPB787R hpaT- (pLHrpF) | UPB787R hpaT- complemented with pLHrpF | This study |
| UPB820R hpaT- (pHpaT) | UPB820R hpaT- complemented with pHpaT | This study |
| UPB820R hpaT- (pHpaH) | UPB820R hpaT- complemented with pHpaH | This study |
| 85-10 | X. euvesicatoria (RfR) | Thieme et al., 2005 |
| 85-10 hrpF- | 85-10 with pOK1 inserted into hrpF | Büttner et al., 2002 |
| 85-10 hrpF- (pHpaT) | 85-10 hrpF - complemented with pHpaT | This study |
Resistances to the antibiotics kanamycin, gentamycin and rifampicin are annotated as KmR, GmR, and RfR, respectively.