Figure 1.

Fortune mandarin CI symptoms and their quantification and schematic diagram of the experimental design used for the analysis of the transcriptome changes. (A) The visual rating scale used to evaluate CI severity. (B) The transcriptomic analysis was performed in the flavedo of freshly harvested fruits (0 d), of the fruits exposed to heat (3 days at 37°C, curing), to cold (20 days at 2°C), and to the heat+cold combination (3 days 37°C+20 d 20°C). The cDNA samples were Cy5-labeled and co-hybridized with a Cy3-labeled cDNA reference pool from a mixture that contained equal amounts of RNA from all the assayed samples.