Figure 3.

3′ RNA processing motifs in zygomycetes, basidiomycetes and fission yeasts. The 3′ termini of the R.oryzae mitochondrial SSU rRNA and of cox1 mRNA were determined by nuclease S1 assays and run on a sequencing gel against a commercial 10 bp ladder (Invitrogen 10821-015) that was 3′ labeled with ddATP (³²P). For experimental details see Materials and Methods. The positions of 3′ termini for both molecules are indicated in the derived consensus sequences by arrows. A small fraction of the undigested form of the SSU rRNA probe is apparent on the gel. In the lower part of the figure, additional, similar motifs in fissions yeasts and basidiomycetes are presented. Uppercase letters indicate 100% conservation and lowercase letters correspond to at least 60% nucleotide conservation. Lowercase Cs between brackets indicate the C-clusters of variable length.