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. 2017 Jun 26;8:1116. doi: 10.3389/fpls.2017.01116

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Copyright © 2017 Wang, Ren, Zhou, Liu, Wang, Xu, Zhang, Zhang, Feng, Wang, Ma, Wang, Guo, Zhang, Lei, Cheng and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sequence analysis and subcellular localization of the WSL4 protein. (A) WSL4 protein has 12 PPR motifs, whereas the wsl4 mutant protein contains only 9. (B) The 12 PPR motifs of WSL4. (C) Transient expression of WSL4–GFP and wsl4–GFP fusion proteins in rice protoplasts. (D) Colocalization of WSL4 (GFP) and PEND (CFP) within chloroplast nucleoids. GFP, GFP signals of WSL4 fusions; CFP, CFP signals of chloroplast nucleoid marker PEND; Auto, Chl autofluorescence; Merged, Merged images of GFP, CFP, and Auto. Bars, 5 μm.