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. 2017 Jun 26;8:1116. doi: 10.3389/fpls.2017.01116

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Copyright © 2017 Wang, Ren, Zhou, Liu, Wang, Xu, Zhang, Zhang, Feng, Wang, Ma, Wang, Guo, Zhang, Lei, Cheng and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Editing efficiencies of rpoB genes in WT and the wsl4 mutant. RT-PCR products of rpoB transcripts from L3-3 of WT, wsl4 mutant (white striped sector), complemented (Com), and RNAi (white striped sector) plants grown in a growth chamber at L30/D25 were sequenced. In wsl4 mutant and RNAi plants, the editing efficiency of rpoB at C545 and C560 were significantly increased compared to WT and Com. The top panel corresponds to the genomic nucleotide sequence. Green, black, red, and blue peak represent A, G, T, and C, respectively. Arrowheads indicate the editing sites and their position in the corresponding chloroplast cDNA strand. L3-3 indicates the third leaf at the three-leaf stage.