Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jan 19;33(2):486–496. doi: 10.1093/nar/gki203

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2005, the authors Nucleic Acids Research, Vol. 33 No. 2 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

PMC Copyright notice

Rat hnRNP A2 has sequence-specific and non-specific sites for binding oligodeoxyribonucleotides. A western blot of protein binding to oligodeoxyribonucleotides and oligoribonucleotides immobilized on superparamagnetic beads in pull-down experiments with brain protein extracts. Heparin was added to the protein extract to suppress non-specific binding. The protein was detected with a polyclonal primary antibody to a peptide from human/mouse hnRNP A2, and an alkaline-phosphatase-conjugated secondary antibody. These oligonucleotides were: the A2RE11 trafficking element (A2RE11 and dA2RE11, the former being the oligoribonucleotide), the AU-rich element (AURE and dAURE), the β-actin mRNA zipcode (ZIP and dZIP) and the non-specific sequence (44,46) (NS1 and dNS1). With the exception of the A2RE11, which binds to a specific site on hnRNP A2, the oligodeoxyribonucleotides but not the oligoribonucleotides bound hnRNP A2.