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. 2005 Jan 19;33(2):486–496. doi: 10.1093/nar/gki203

Figure 3.

Figure 3

The telomeric repeat competes for the hnRNP A2 dA2RE11 binding site. Competition UV-cross-linking EMSAs in which 1.5 pmol 32P-labeled dA2RE11, dNS1 or telomeric sequences (Telo1 and Telo3) were incubated with 10 pmol of purified recombinant hnRNP A2 in the presence or absence of 75 pmol unlabeled competitor, irradiated, electrophoresed on a 15% SDS–polyacrylamide gel and autoradiographed. Telo1 (A) contains a single telomeric repeat and Telo3 (B) oligonucleotide has three repeats of the telomeric sequence [i.e. (TTAGGG)3]. (C) Competition UV-cross-linking EMSAs performed by incubating 32P end-labeled rA2RE11 and Telo1 with recombinant hnRNP A2 (10 pmol) in the presence or absence of unlabeled competitor, as above. The telomeric DNA repeat sequence competes with dA2RE11, but weakly with A2RE11, for the binding site on recombinant hnRNP A2.