Figure 3.
Glycan screening and binding analysis by SPR. (a) The region corresponding to the top hits on a binding screen with 609 different glycans attached on a microarray is enriched on sulfated oligosaccharides. The sulfated glycans were classified according to the position of the sulfate group. Position 6, position 4 and position 3 are indicated by the purple, green and orange bars, respectively. (b) Kinetics of interaction between FraC and chondroitin sulfate C determined by SPR. The chondroitin sulfate C was immobilized on a chip and FraC was flowed at 0.5, 1.0, 2.0, 4.0, 8.0, 16.1, 32.2, 64.4 and 128.7 µM. The first and second arrow indicates the interval when FraC is accessing the sensor-chip where the polysaccharide was immobilized. Data were fit to single-site model to calculate kinetic parameters. (c) Determination of the equilibrium constant from the binding response in equilibrium (KD = 24 µM).