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. 2005 Jan 28;33(2):672–682. doi: 10.1093/nar/gki214

Figure 2.

Figure 2

MUTYH does not form a stable complex with duplex oligonucleotides containing 2-OH-A opposite guanine. 20 nM duplex oligonucleotides containing *A:GO (lanes 1, 3 and 8), *AO:G (lanes 2, 4 and 9), *AO:GO (lanes 5 and 10), *A:G (lanes 6 and 11) or *AO:T pairs (lanes 7 and 12) were incubated with 80 nM wild-type mMUTYH (lanes 3–7) or mutant mMUTYH(D207N) (lanes 8–12) at 37°C for 60 min. The reaction products were fractionated on a 4% native polyacrylamide gel. Lanes 1 and 2, no protein; free, unbound 35mer duplex; bound, MUTYH-bound duplex. *, base in the FAM-labeled strand.