Table 2.

Kinetic parameters of wild-type mMUTYH and mutant mMUTYH(G365D)

	Apparent Vmax(nM s−1) × 10−2	Apparent Km(nM)	kcat/Km (nM−1s−1) × 10−5
*A:GO (4°C)
Wild	1.12	15.37	1.82
G365D	1.02 (0.91)	16.50 (1.11)	1.55 (0.85)
*AO:G (37°C)
Wild	1.53	2.34	16.37
G365D	0.21 (0.14)	21.95(9.38)	0.24 (0.015)

Values of apparent V_max and apparent K_m of wild-type mMUTYH and mutant mMUTYH(G365D) for each substrate containing *A:GO and *AO:G pairs were calculated from Figure 7. The values of k_cat were calculated as the molecules of the product formed per molecule of MUTYH per second. The values in parentheses are the relative ratios to those of wild-type mMUTYH.

^*, base in the FAM-labeled strand.