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Rate constants and dissociation constants of RNA aptamers against domain IIId of HCV IRES
| RNA aptamer | kon (×104 M−1s−1) | koff (×10−4 s−1) | KD (nM) |
|---|---|---|---|
| 3-01 | 19 ± 10 | 5.4 ± 1 | 4.4 ± 3 |
| 3-03 | 1.1 ± 0.6 | 3.5 ± 1 | 43 ± 30 |
| 3-04 | 6.0 ± 1 | 7.2 ± 2 | 12 ± 2 |
| 3-05 | 10 ± 0.9 | 7.2 ± 0.1 | 7.2 ± 0.7 |
| 3-06 | 6.1 ± 3 | 6.5 ± 3 | 13 ± 9 |
| 3-07 | 5.9 ± 2 | 5.1 ± 2 | 9.6 ± 4 |
| 3-15 | ND | ND | ND |
| 3-17 | 1.8 ± 0.4 | 23 ± 2 | 130 ± 30 |
| 3-18 | 1.3 ± 1 | 2.7 ± 2 | 22 ± 4 |
ND: binding response was not detected.
