Table 1.
Oligonucleotides used in this study
| Oligomers and their length (nt) | Method of synthesis |
|---|---|
| X(+) (98) | In vitro transcription; primers: D1 (taatacgact cactataggt ggctccatct tagccctagt cacggctagc tgtgaaaggt ccgtgagccg c) and D2 (acatgatctg cagagaggcc agtatcagca ctctctgcag tcatgcggct cacggacc) |
| SL3 (20) | In vitro transcription; primers: T7 (taatacgact cactatag) and D4 (agggctaaga tggagccacc tatagtgagt cgtatta) |
| SL2 (31) | In vitro transcription; primers: T7 (taatacgact cactatag) and D5 (gctcacggac ctttcacagc tagccgtgac ctatagtgag tcgtatta) |
| SL2ab (33) | Chemical synthesis |
| X(−) (99) | In vitro transcription; primers: D6 (taatacgact cactataggc atgatctgca gagaggc) and D7 (ggtggctcca tcttagc); template: DX (taatacgact cactataggt ggctccatct tagccctagt cacggctagc tgtgaaaggt ccgtgagccg catgactgca gagagtgctg atactggcct ctctgcagat catgt) |
| SL3(−) (24) | In vitro transcription; primers: D10 (taatacgact cactatagga gggctaagat ggagccactc c) and D11 (ggagtggctc catcttagcc ctcctatagt gagtcgtatt a) |
| SL2(−) (27) | Chemical synthesis |