Table 3.

mRNA primer sequences used for RT-PCR ^a.

Gene	Forward Primer	Reverse Primer
CRHR1	5′-TTCACCTCCCTTCAGGATCA-3′	5′-TGCAGGCCAGAAACATTGC-3′
CRHR2insoluble	5′-TTTGGATGACAAGCAGAGGAAGT-3′	5′-GCACTAGGAAAAGCAGGAAAGC-3′
CRHR2soluble	5′-CCCATTTTGGATGACAAGGAGTA-3′	5’-GGATGAAGGTGGTGATGAGGTT-3′
CRH	5′-CAGAACAACAGTGCGGGCTCA-3′	5′-AAGGCAGACAGGGCGACAGAG-3′
POMC	5′-GAGATTCTGCTACAGTCGCTC-3′	5′-TTGATGATGGCGTTCTTGAA-3′
PPO	5′-CATCCTCACTCTGGGAAAG-3′	5′-AGGGATATGGCTCTAGCTC-3′
OR1	5′-GCGATTATCTCTATCCGAAGC-3′	5′-CAGGGACAGGTTGACAATG-3′
OR2	5-′TGTTCAAGAGCACAGCCAAACG-3′	5′-GCCAATACCATAAGACACAGGGG-3′
GAPDH	5′-AAGGGCTCATGACCACAGT-3′	5′-GGATGCAGGGATGATGTTCT-3′
Β-actin	5′-TTGCTGACAGGATGCAGAA-3′	5′-ACCAATCCACACAGAGTACTT-3′

^a CRHR: corticotropin releasing hormone receptor, CRH: corticotropin releasing hormone, POMC: pro-opiomelanocortin, PPO: prepro-orexin, OR: orexin receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase. CRH receptor subtype and GAPDH primers were developed based on sequences from [17]. CRH and β-actin primers were based on sequences derived from [22]. POMC primers were derived from [18]. Pre-pro-orexin, OR1, OR2, primers were derived from C. Kotz (personal communication).