Skip to main content
. 2005 Feb 1;33(2):796–804. doi: 10.1093/nar/gki220

Figure 1.

Figure 1

HIV-1 escape variants that resist siRNA-Nef inhibition. (A) Schematic representation of the HIV-1 LAI proviral genome. The position of the siRNA-Nef target sequence is indicated with an arrow. This sequence is present in both the unspliced and all spliced forms of HIV-1 RNA. (B) HIV-1 LAI variants resistant to siRNA-Nef were selected in nine independent cultures. The Nef target sequence (gray box indicates nucleotides 164–182 of the Nef gene; nucleotides 8553–8571 in the LAI RNA genome) and flanking sequences are shown for the wild-type (LAI) and the evolved RNAi-resistant viruses (R1–R9). The day at which the escape variants were sequenced is indicated. Deletions are shown as dashes, substitutions are underlined and in bold. In the R1 virus, nucleotides 125–230 of the Nef gene are deleted. In the R5 virus, nucleotides 179–241 are deleted. In the R7 virus, we observed deletion of nucleotides 44–268 and a T269A substitution.