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. 2005 Feb 1;33(2):755–764. doi: 10.1093/nar/gki225

Figure 3.

Figure 3

The effects of alanine substitutions in the β subunit on the displacement of RNAP by Mfd in vitro. Displacement of stalled transcription complexes from radiolabelled DNA was monitored by EMSA, and quantified using a phosphorimager and ImageQuant software. Data points represent the amount of intact elongation complex present in each sample, expressed as a percentage of the amount present prior to the addition of Mfd (t = 0). Displacement assays were carried out on wild-type RNAP (open circles), RNAP βIA117 (filled squares), RNAP βKA118 (filled triangles) and RNAP βEA119 (filled circles). Values are the average of at least three independent experiments (with SD). (A) Time course of displacement of stalled RNAP at 37°C by 250 nM Mfd. (B) Effect of Mfd concentration on displacement of stalled RNAP. Samples were incubated with Mfd for 5 min at 37°C.