Table 1.
Bacterial strains and plasmids used in this work
| Strains | Genotype | Source or reference |
|---|---|---|
| E.coli | ||
| DH5α | F- φ80dlacZΔM15 Δ(lacZYA-argF) U169 deoR recA1 endA1 hsdR17 (rK−mK+) phoA supE44 λ-thi-1 gyrA96 relA1 | Gibco, BRL |
| M.tuberculosis H37Rv | ATCC 25618 | |
| M.smegmatis mc2155 | (54) | |
| Plasmids | ||
| pMS2 | Hygr; pAL500 origin, ColE1 origin, contains multiple cloning site and 2 transcriptional terminators | (25) |
| pMGuvK | pMS2 derivative, Hygr, promoterless gfpuv, promoterless Kmr | This work and (25) |
| pMGK | pMS2 derivative, Hygr, promoterless gfp+, promoterless Kmr | This work and (24) |
| pWH520 | (27) | |
| pUV15 | pMS2 derivative, Hygr, Kmr, psmyc-gfp | This work and (25) |
| pUV15tetORs | pUV15 derivative, Hygr, Kmr, psmyc-tetR, pmyc1tetO-gfp+ | This work |
| pUV15tetORm | pUV15 derivative, Hygr, Kmr, pimyc-tetR, pmyc1tetO-gfp+ | This work |
| pME0L0 | pMS2 derivative, Hygr, promoterless lacZ | This work |
| pME0L1 | pMS2 derivative, Hygr, pmyc1tetOlacZ | This work |
| pME1sL1 | pMS2 derivative, Hygr, psmyc-tetR(B), pmyc1tetO-lacZ | This work |
| pME1mL1 | pMS2 derivative, Hygr, pimyc-tetR(B), pmyc1tetO-lacZ | This work |
| pMV306Km | Kmr, int, attP integrates at attB site on mycobacterial chromosome | (32) |
| pMC1s | pMV306Km derivative, Kmr, psmyc-tetR | This work |