Skip to main content
. 2005 Feb 1;33(2):e23. doi: 10.1093/nar/gni021

Figure 2.

Figure 2

ExoIII digestion of the FRET probe. (A) Emission spectra of free NF-κB probe digested by ExoIII. Curve 1, the 25 nM NF-κB probe digested by ExoIII (ExoIII-positive control); Curve 2, the 25 nM NF-κB probe not digested by ExoIII (ExoIII-negative control). (B) Emission spectra of p50-bound NF-κB probe digested by ExoIII. Insets are gel electrophoresis and UV transilluminator images. (C) Dynamics of ExoIII digestion of NF-κB probes. In (B) and (C), curve 1, the ExoIII-positive control of 25 nM NF-κB probe; curve 2, the 25 nM NF-κB probe bound by 15 nM p50 and digested by ExoIII; curve 3, the ExoIII-negative control of the 25 nM NF-κB probe.