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. 2005 Feb 1;33(2):e23. doi: 10.1093/nar/gni021

Figure 3.

Figure 3

Specificity of protein detection. (A) Emission spectra of the 25 nM NF-κB probes in the presence of 20 nM NF-κB p50 and increasing amounts of cold specific probe. (B) Emission spectra of the 25 nM NF-κB FRET probes in the presence of 20 nM NF-κB p50 and increasing amounts of cold nonspecific probe. (C) Relative FRET of the competition assay. The final concentrations of the competitor probe were 0 nM (6 and 6′), 10 nM (5 and 5′), 20 nM (4 and 4′), 40 nM (3 and 3′) and 80 nM (2 and 2′). ExoIII-positive and ExoIII-negative controls of the 25 nM NF-κB probe were (1 and 1′) and (7 and 7′), respectively.