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Sequence-dependent cytotoxicity of the combination treatment of ICG-PDT and VP-16 in A549 cells. Cells were incubated with ICG for 4 h and then irradiated at 800 nm. VP-16 was added after laser irradiation. Cell viability was measured by the standard MTT assay. The solid lines are best fits to the experimental data using a logistic function in Origin software.
