TABLE 1.
Target | Linearity |
Accuracy (sensitivity %) |
Reproducibility (CV%) |
|||
---|---|---|---|---|---|---|
Linearity (R2) | Efficiency (%) | High concn | Low concn | High concn | Low concn | |
Bartonella | 0.994 | 99.4 | 100 | 80.0 | 1.6 | 2.3 |
Chikungunya 1 virus | 0.991 | 99.1 | 100 | 100 | 1.3 | 5.7 |
Chikungunya 2 virus | 0.994 | 99.4 | 100 | 100 | 1.8 | 5.5 |
CMV | 0.994 | 99.4 | 100 | 100 | 1.5 | 4.1 |
EBV | 0.987 | 98.7 | 100 | 100 | 1.5 | 6.8 |
HSV 1 | 0.987 | 98.7 | 96.7 | 100 | 2.3 | 4 |
HSV 2 | 0.993 | 99.3 | 100 | 100 | 0.9 | 4 |
Measles virus | 0.92 | 92.0 | 100 | 55.0 | 8.6 | 6.7 |
Mumps virus | 0.997 | 99.7 | 100 | 100 | 1.1 | 2 |
Mycobacterium tuberculosis | 0.993 | 99.3 | 96.7 | 100 | 1.0 | 5.1 |
VZV | 0.993 | 99.3 | 100 | 95.0 | 2.4 | 2.7 |
Mycoplasma pneumoniae | 0.993 | 99.3 | 100 | 100 | 1.0 | 2.9 |
Nipah virus | 0.980 | 98.0 | 100 | 100 | 1.4 | 1 |
Neisseria meningitidis | 0.994 | 99.4 | 100 | 100 | 1.8 | 4 |
Pan-dengue virus | 0.993 | 99.3 | 100 | 100 | 2.1 | 1.7 |
Haemophilus influenza | 0.993 | 99.3 | 100 | 100 | 1.2 | 5.2 |
Parechovirus | 0.995 | 99.5 | 96.6 | 85.0 | 1.9 | 5.6 |
Streptococcus pneumoniae | 0.992 | 99.2 | 100 | 95.0 | 1.2 | 7.4 |
RNase P | 0.993 | 99.3 | 100 | 95.0 | 1.8 | 1.6 |
Acanthamoeba | 0.990 | 99.0 | 100 | 100 | 1.8 | 4.1 |
Balamuthia mandrillaris | 0.990 | 99.0 | 100 | 100 | 1.7 | 5.4 |
High concentration = 1.6 × 10−2 ng/well or 5.3 × 106 copies per well; low concentration = 1.6 × 10−7 ng/well or 54 copies per well. CV, coefficient of variance.