Identification of C. coli genomes unassigned to a clonal complex from clinical (n = 42) (black triangles) and nonclinical (n = 175) (gray triangles) isolates belonging to clades 1, 2, and 3. Concatenated sequences from seven-locus MLST alleles of isolates used to validate the human disease cgMLST scheme v1.0 were used to construct a neighbor-joining tree, which included reference isolates of known C. coli clades that are colored as follows: red, C. coli clade 1; yellow, C. coli clade 2; green, C. coli clade 3. Reference isolates are detailed in Table S2. The scale bar represents the p-distance between aligned sequences.