TABLE 1.

Mutations determined by the GeneXpert MTB/RIF assay, the Hain MTBDRplus test, and sequencing of the individual genes, performed with leftovers of the Xpert processed sputum specimens

Sample	Patienta		GeneXpert result		rpoB result		MDR	katG result (codon 315)		inhA promoter result (position −15)		Sequencing result for:
	Sex	Age (yr)	Probe(s)b	Mutation(s) detected at codon(s):	Sequencingc	Hain test		Sequencing	Hain test	Sequencing	Hain test	pncA	rrs	rpsL	gyrA
GAB-001	M	54	B and C	516 or 522	D516Y	Mutated (probes WT4 and -5 absent)	x	S315T	S315T	WT	WT	WT			WT
GAB-065	F	19	B and C	516 or 522	D516Y	Mutated (probe WT4 absent)	x	S315T	S315T	WT	WT	WT			WT
GAB-152	F	18	B	516	D516Y	Mutated (probe WT4 absent)	x	S315T	S315T	WT	WT	WT			WT
GAB-076	F	26	C	522	D516Y, S522T	Mutated (probe WT4 absent)	x	S315T	S315T	WT	WT	WT			WT
GAB-163	F	23	E	531 or 533	NS	S531L	x	S315T	S315T	WT	WT	NS			WT
GAB-003	M	35	E	531 or 533	S531L	S531L	x	S315T	S315T	WT	WT	WT			WT
GAB-009	F	37	E	531 or 533	S531L	S531L	x	S315T	S315T	WT	WT	G97S			WT
GAB-182	M	32	E	531 or 533	S531L	S531L	x	S315T	S315T	WT	WT	T135P			WT
GAB-002	F	50	B	516	D516Y	Mutated (probe WT4 absent)	x	S315T	S315T	WT	C → T	WT	WT	K43R	D94G
GAB-180	M	19	B	516	D516Y	Mutated (probe WT4 absent)	x	S315T	S315T	WT	C → T	WT			WT
GAB-014	M	30	E	531 or 533	NS	Mutated (probe WT8 absent)	x	WT	WT	C → T	C → T	NS	WT		NS
GAB-173	M	44	E	531 or 533	S531L	S531L	x	WT	WT	C → T	C → T	WT	WT	WT	WT
GAB-062	M	18	E	531 or 533	NS	S531L	x	WT	WT	C → T	C → T	WT			WT
GAB-151	F	42	E	531 or 533	NS	S531L	x	WT	WT	C → T	C → T	WT			WT
GAB-157	M	25	E	531 or 533	NS	S531L	x	WT	WT	C → T	C → T	WT			WT
GAB-010	F	28	E	531 or 533	S531L	S531L	x	WT	WT	C → T	WT	S88Stop			WT
GAB-068	F	54	E	531 or 533	NS	S531L	x	NS	S315T	WT	WT	G97S			WT
GAB-072	M	23	C	522	NS	Mutated (probes WT3 and -4 absent)	x	NS	S315T	NS	WT	WT			WT
GAB-017	M	26	E	531 or 533	S531L	S531L		WT	S315T	WT	WT	NS			WT
GAB-059	F	32	B	516	NS	WT		NS	WT/S315Td	WT	WT	WT			WT
GAB-191	?	?	B	516	NS	TUBe control absent		WT	WT	NS	C → T	NS			WT

^aM, male; F, female; ?, unknown.

^bXpert probe B can also detect less-frequent mutations affecting amino acid 513 and a deletion of amino acids 516 and 517 (31).

^cNS, PCR amplification was not successful.

^dMixed profile; see Results.

^eTUB, M. tuberculosis complex specific.