TABLE 4.
Primers used to amplify and sequence the Mycobacterium tuberculosis genes associated with resistance to antibiotics
| Gene | Antibiotic | Primer name | Primer sequence (5′–3′) | Hybridization temp (°C) | Product size (bp) |
|---|---|---|---|---|---|
| rpoB | Rifampin | TR1 | TACGGTCGGCGAGCTGATCC | 53 | 411 |
| TR2 | TACGG CGTTTCGATGAACC | ||||
| katG | Isoniazid | katG1 | TGGCCGCGGCGGTCGACATT | 60 | 330 |
| katG2 | CCAGCAGGGCTCTTCGTCAG | ||||
| inhA | Isoniazid | inhA promo-1 | CCTCGCTGCCCAGAAAGGGA | 60 | 248 |
| inhA promo-2 | ATCCCCCGGTTTCCTCCGGT | ||||
| pncA | Pyrazinamide | pncA1 | ATCGCGATGGAACGTGATA | 60 | 950 |
| pncA2 | CTGTCACCGGACGGATTTG | ||||
| gyrA | Fluoroquinolones | gyrA-F | GATGACAGACACGACGTTGC | 55 | 398 |
| gyrA-R | GGGCTTCGGTGTACCTCAT | ||||
| gyrB | Fluoroquinolones | gyrB-F | CCACCGACATCGGTGGATT | 54 | 427 |
| gyrB-R | CTGCCACTTGAGTTTGTACA | ||||
| rrs | Aminoglycosides (capreomycin) | rrs-F | AAACCTCTTTCACCATCGAC | 59 | 1,329 |
| rrs-R | GTATCCATTGATGCTCGC | ||||
| rpsL | Streptomycin | rpsLfw | GGCCGACAAACAGAACGT | 51 | 501 |
| rpsLrev | GTTCACCAACTGGGTGAC |