TABLE 5.
Key features of the principal HTLV-1/2 screening assays used in the studya
| Assay name (reference[s]) | Mfr | Assay type | Analyzer system | Key principle of assay | Testing time (min) |
|---|---|---|---|---|---|
| Elecsys HTLV-I/II (19) | Roche Diagnostics | One-step double-antigen sandwich chemiluminescent immunoassay | Modular Analytics E170 or cobas e 411, cobas e 601, cobas e 602 | Detects antibodies to viral recombinant antigens gp21 and p24 | 18 |
| Abbott Architect rHTLV-I/II (21) | Abbott Laboratories | Two-step chemiluminescent immunoassay | Architect i system | Detects antibodies to viral synthetic peptide gp46 and recombinant antigen gp21 | 29 |
| Ortho Avioq HTLV-I/II Microelisa system (24) | Avioq Inc. | ELISA | Microelisa plate reader | Detects antibodies to viral antigens (purified viral lysate) and recombinant HTLV-1 p21E antigen | 150 |
| Abbott Prism HTLV-I/HTLV-II (23, 27) | Abbott Laboratories | Three-step sandwich chemiluminescent immunoassay | Abbott Prism system | Detects antibodies to HTLV-1 and HTLV-2 antigens | 54 |
| DiaSorin Murex HTLV I+II (28) | DiaSorin | ELISA | Microelisa plate reader | Detects antibodies to HTLV-1 and HTLV-2 antigens | 90 |
a
Abbreviations: Mfr, manufacturer; ELISA, enzyme-linked immunosorbent assay.