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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Methods Mol Biol. 2017;1596:131–145. doi: 10.1007/978-1-4939-6940-1_9

Fig. 4.

Fig. 4

In vivo measurement of ATM kinase activity in mouse tumor xenograft model. (a) CD-1 nude mice harboring D54-ATMR WT tumor xenografts were injected with luciferin and bioluminescence was acquired as described 3 h before treatments. (b) The animals were injected with KU-55933 (25 mg/kg) or vehicle control (DMSO) and bioluminescence was acquired 1,4, 8, and 24 h posttreatment. The ATM reporter fold activation upon ATM inhibition is plotted over mock treatment. (c) Similarly, mouse harboring D54-ATMR WT tumor xenografts were whole body irradiated with 5Gy of radiation or sham irradiated and bioluminescence was measured for up to 24 h. About 70% decrease in the reporter activity was observed 8 h post irradiation