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. 2017 Jun 26;8:708. doi: 10.3389/fimmu.2017.00708

Figure 1.

Figure 1

The Δomp25 Brucella suis mutant induces interleukin (IL)-12 production in human THP-1 cells. (A) THP-1 cells were infected with wild-type (WT) B. suis, Omp25 mutant (Δomp25) or were uninfected (Ctrl) as described in Section “Materials and Methods,” and cells were cultured for 24 h in complete culture medium. The concentrations of IL-12 p70 in cell supernatants were determined by ELISA. (B) THP-1 cells were infected as in (A) and cultured for 6 h, followed by quantitative polymerase chain reaction detection of il12A (p35) and il12B (p40) mRNAs levels in THP-1 cells. (C) THP-1 cells were infected with 100 multiplicity of infection of recombinant adenoviruses expressing Omp25 protein (rAd-Omp25) or rAd-Blank for 24 h and then infected with Δomp25 for another 24 h or uninfected (Ctrl), followed by ELISA detection of IL-12 p70 in cell supernatants. Each value represents means ± SEMs of three independent experiments. ##P < 0.01 versus WT B. suis-infected cells. *P < 0.05, **P < 0.01 versus rAd-Blank-infected cells in same treatment.