Fig. 4. Genome-wide and targeted RNAi in EWS cell lines.

(A) A genome-wide functional screen was performed on the EWS cell line CHP100 using the Sigma Aldrich human genome library (~5 shRNAs per gene). 627 genes (5.3%) were scored as positive hits based on the criteria of ≥ 80% of shRNAs per gene resulting in threshold reduction of nuclei count. (B) Genes encoding 26S proteasome subunits or 19S-associated DUBs were significantly over-represented as positive hits.