Table 2.

MicroRNAs profiled in the assay

MicroRNA*	Sequence†	Forward primer sequence‡
hsa-miR-31-5p	AGGCAAGATGCTGGCATAGCT	AGGCAAGATGCTGGCATAGCT
hsa-miR-5701	TTATTGTCACGTTCTGATT	AGTCATTTGGCTTATTGTCACGTTCTGATT
hsa-miR-424-3p	CAAAACGTGAGGCGCTGCTAT	CAAAACGTGAGGCGCTGCTAT
MID-50971	ATACTCTGGTTTCTTTTC	CAGTCATTTGGCATACTCTGGTTTCTTTTC
MID-20094	TAAGCCAGTTTCTGTCTGATA	CATTTGGCTAAGCCAGTTTCTGTCTGATA
MID-50976	CTGTCTGAGCGCCGCTC	CCTGTCTGAGCGCCGCTC
hsa-miR-3074-5p	GTTCCTGCTGAACTGAGCCAG	CGTTCCTGCTGAACTGAGCCAG
hsa-miR-222-3p	AGCTACATCTGGCTACTGGGT	GCAGCTACATCTGGCTACTGGGT
MID-50969	ATGACAGATTGACATGGACAATT	TGGCATGACAGATTGACATGGACAATT
hsa-miR-146b-5p	TGAGAACTGAATTCCATAGGCT	TGGCTGAGAACTGAATTCCATAGGCT
hsa-miR-346	TGTCTGCCCGCATGCCTGCCTCT	TGTCTGCCCGCATGCCTGCCTCT
MID-16582	AGTGAAGCATTGGACTGTA	TTGGCAGTGAAGCATTGGACTGTA
hsa-miR-342-3p	TCTCACACAGAAATCGCACCCGT	CAGTCATTTGGGTCTCACACAGAAATCG
hsa-miR-181c-5p	AACATTCAACCTGTCGGTGAGT	CAGTCATTTGGCAACATTCAACCTGTCG
hsa-miR-125b-5p	TCCCTGAGACCCTAACTTGTGA	CAGTCATTTGGGTCCCTGAGACCCTAAC
hsa-miR-375	TTTGTTCGTTCGGCTCGCGTGA	CAGTCATTTGGGTTTGTTCGTTCGGCTC
hsa-miR-486-5p	TCCTGTACTGAGCTGCCCCGAG	CAGTCATTTGGCTCCTGTACTGAGCTGC
hsa-miR-551b-3p	GCGACCCATACTTGGTTTCAG	CAGTCATTTGGCGCGACCCATACTTGGT
hsa-miR-23a-3p	ATCACATTGCCAGGGATTTCC	CAGTCATTTGGCATCACATTGCCAGGGA
hsa-miR-574-3p	CACGCTCATGCACACACCCACA	CAGTCATTTGGCCACGCTCATGCACACA
hsa-miR-152-3p	TCAGTGCATGACAGAACTTGG	CAGTCATTTGGCTCAGTGCATGACAGAA
hsa-miR-200c-3p	TAATACTGCCGGGTAATGATGGA	CAGTCATTTGGGTAATACTGCCGGGTAA
hsa-miR-138-5p	AGCTGGTGTTGTGAATCAGGCCG	CAGTCATTTGGCAGCTGGTGTTGTGAAT
hsa-miR-345-5p	GCTGACTCCTAGTCCAGGGCTC	CAGTCATTTGGCGCTGACTCCTAGTCCA

*microRNA names that begin with ‘hsa’ are in miRBase, those that begin with ‘MID’ were sequenced and/or predicted at Rosetta Genomics.

†microRNA sequence is from miRBase²⁸ V.20.

‡Reverse primer sequence: GCGAGCACAGAATTAATACGAC.