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. Author manuscript; available in PMC: 2017 Jun 26.
Published in final edited form as: Biomaterials. 2017 Mar 6;128:19–32. doi: 10.1016/j.biomaterials.2017.03.002

Figure 5. (PVPON/TA) multilayers can elicit a decrease in the extracellular M1 macrophage marker CD80 with p(I:C)-stimulated macrophages.

Figure 5

Flow cytometric analysis of CD80 within p(I:C)-stimulated BM-Mϕs in the presence or absence of (PVPON/TA) capsules for 24 hours (A). Macrophages were gated using forward scatter and side scatter profiles, doublets were excluded, and live cells were gated using side scatter profiles using a fixable live/dead stain, immunophenotyped by F4/80, and CD80 cell surface marker activation shown via percentage and geometric mean fluorescence intensity (gMFI). Pooled CD80 percentages represent 3 independent experiments done in triplicates depicting the percentage of CD80 expressing cells gated on the F4/80 population (B). ns, not significant; **p<0.01.