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. Author manuscript; available in PMC: 2017 Jun 26.
Published in final edited form as: Cell Rep. 2017 Apr 25;19(4):671–679. doi: 10.1016/j.celrep.2017.04.001

Figure 1. Tip60 KAT and p400 ATPase activities are dispensable for ESC self-renewal and gene regulation.

Figure 1

(A) Alkaline phosphatase staining (AP) of Tip60ci/ci and Ep400ci/ci mutants and controls (Tip60fl/+, Tip60 KD, Ep400 KD, and Ep400hypo). Scale bars equal 200 μm. (B) Growth curve, measuring the proliferation rates of the indicated mutant and control ESCs. (C, D) Heatmaps of differentially expressed genes in Tip60ci/ci and Tip60 KD ESCs relative to Tip60fl/+control cells (C), or Ep400ci/ci and Ep400hypo ESCs relative to wild type (E14) control ESCs (D). Genes in the heatmaps are sorted from the most upregulated to the most down regulated genes in the Tip60 KD and Ep400hypo controls, respectively. (E, F) Venn diagrams showing number of genes commonly misregulated in Tip60ci/ci and Tip60 KD ESCs (E), or Ep400ci/ci and Ep400hypo ESCs (F). Genes were considered significantly misregulated in each KD or mutant if their |log2 (fold change)| > 1 and their multiple testing-adjusted p value < 0.05.