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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Nitric Oxide. 2017 Apr 5;66:62–70. doi: 10.1016/j.niox.2017.04.001

Figure 1. Ex-vivo nitrate reductase (NR) activity of human posterior tongue.

Figure 1

Panel A: Tongue scrapes were collected from 3 volunteers and incubated for 10min in BHI broth, 37°C. Nitrate (at indicated doses) was then added and nitrite formation measured after 40min. Data are mean ± SEM (n=4, except for nitrate doses 200–800μM, where n=3). Panel B: Average time-dependent nitrite formation (after nitrate addition) in male (□) and female (●) samples. Data are mean ± SEM (n=5 (female) and n=4 (male)), Panel C: Average time-dependent nitrite formation (after nitrate addition) normalized to CFU in male and female samples. Data are mean ± SEM (n=5 (female) and n=4 (male)) *P<0.05 by 2-way ANOVA. Panel D: Nitrate reduction to nitrite was measured in paired tongue scrapes collected from the front and back of the tongue. Data show mean ± SEM (n=5 different donors). *P<0.05 by 2-way ANOVA and #P<0.05 by Sidak’s Multiple comparison test. Panel E: data from Panel D normalized to CFU.