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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Nitric Oxide. 2017 Apr 5;66:62–70. doi: 10.1016/j.niox.2017.04.001

Figure 4. Effects of chlorohexidine on nitrate reductase activity in mice.

Figure 4

Chlorohexidine (0.2%) was administered directly onto the posterior tongue in a 10μl volume either once daily, 7d, or twice daily 3d or 7d and then nitrate reductase activity measured (Panel A), CFU measured after 18h culture in BHI (Panel B) and initial rate of nitrate reductase activity normalized to CFU calculated (Panel C). Data are mean ± SEM (n=3 for 3 days twice daily; n=3 for 7 days once daily; n=6 for 7 days twice daily, n=7 for vehicle control). Panel D shows CFU counts from vehicle (water) or chlorohexidine treated mice (2× day, 7days), upon collection of tongue swabs or after 18 culturing in BHI broth. Data show mean ± SEM (n=5 in each group). *P<0.05 by unpaired t-test. Two data points from 7 days twice daily and one data point from vehicle control were excluded by ROUT analysis.