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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Nitric Oxide. 2017 Apr 5;66:62–70. doi: 10.1016/j.niox.2017.04.001

Figure 5.

Figure 5

Male C57Bl/6 male mice received water or water supplemented with nitrate for 2 weeks. Chlorohexidine (0.2%) was administered directly onto the posterior tongue in a 10μl volume twice daily for the last 7d. Panel A: Oral nitrate reductase activity was measured as described in Figure 3. Data show nitrite levels 20min after addition of nitrate to tongue swabs Panel B: CFU measured after 18h culture in BHI. Panel C: Initial rate of nitrate reductase activity normalized to CFU. All data are mean ± SEM (H2O n=8, CHX n=8, Nitrate-H2O n=7, Nitrate-CHX n=5). *p<0.05 by unpaired t-test; #p<0.05 by 1-way ANOVA with Tukey post-test. One outlier from Nitrate-H2O and two outliers from nitrate-CHX removed by ROUT analysis. Panel D shows the nitrite levels in plasma. Data are mean ± SEM (n=8 except for H2O group where n=9). *p<0.05 by 1-way ANOVA with Tukeys post-test.