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. 2017 Jun 2;15(6):161. doi: 10.3390/md15060161

Figure 1.

Figure 1

Affinity chromatography of Aplysia kurodai lectin (AKL). (a) affinity purification of AKL. The crude egg mass extract was applied to a d-galactose-Sepharose 6B affinity column. Unbound proteins were eluted by washing with 20 mM Tris-HCl, pH 8.0, 0.15 M NaCl (the first peak), and the bound lectin was eluted by 0.2 M d-galactose (the second peak, bar); (b) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of purified AKL under non-reducing conditions (lane 1), reducing conditions (lane 2), and molecular standards (M).