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. Author manuscript; available in PMC: 2018 Apr 20.
Published in final edited form as: Cell Chem Biol. 2017 Apr 6;24(4):481–492.e5. doi: 10.1016/j.chembiol.2017.03.008

Figure 3.

Figure 3

Eg5 interacts with HDAC1. A) Wild type (WT) or C151A HDAC1 were expressed as FLAG-tagged proteins in T-Ag Jurkat cells, immunoprecipitated (IP) with anti-FLAG agarose in the absence (lanes 2 and 3) or presence (lane 4) of SAHA (3 mM), separated by SDS-PAGE, and Western blotted with Eg5 (top), acetyl-lysine (AcLys, middle), or FLAG (bottom) antibodies. B) Endogenous HDAC1 and Eg5 were immunoprecipitated (IP) separately from T-Ag Jurkat cells, separated by SDS-PAGE, and Western blotted with Eg5 (top) or HDAC1 (bottom) antibodies. C) HDAC1-FLAG and Eg5-myc were coexpressed in HEK293 cells, immunoprecipitated (IP) separately with either FLAG or myc antibodies, separated by SDS-PAGE, and Western blotted with myc (top) or FLAG (bottom) antibodies. Agarose beads without bound antibodies were used as an immunoprecipitation controls (lane 1). For all gels, arrows indicate the position of Eg5 and HDAC1 in the gels.