Figure 3.

(A) Ca²⁺ mobilization assay CRC for 27 in HEK293A cells expressing human mGlu₅. (B) Effects of 27 on glutamate CRC in rat cortical astrocytes. (C) Competition radioligand binding CRC with 27 and [³H]-3-methoxy-5-(pyridin-2-ylethynyl)pyridine (39). (D) Saturation binding experiment: increasing concentrations of [³H]-39 in the presence and absence of multiple concentrations of 27. (E) Saturation binding experiment: Scatchard analysis of bound/free versus bound ligand. (F) Effects of the neutral mGlu₅ site ligand 5-methyl-6-(phenylethynyl)-pyridine (40) on the functional inhibition of glutamate responses induced by 27.