Figure 6. Example recording of hiPSC-RGCs co-cultured with SC slices.

A. Whole-cell voltage clamp recording of I_Na and I_K in response to step depolarizations (150 msec, -66 mV to +24 mV from a holding potential of -76 mV). I_Na is blocked by 1 mM TTX. B. Expanded time axis of current responses to a depolarizing step to -26 mV in control conditions and in the presence of 1 mM TTX. C. Current-clamp recording showing voltage responses to hyperpolarizing and depolarizing current injections (-20 to +50 pA). The cell fires action potentials in response to depolarizing current injections.