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. 2005 Mar;79(5):2743–2753. doi: 10.1128/JVI.79.5.2743-2753.2005

FIG. 10.

FIG. 10.

σ1s is expressed in vivo following both intramuscular and intracranial inoculation with 105 PFU of T3A (σ1s+ SA+) (A and G) and C84 (σ1s+ SA−) (B and H), but not C84-MA (σ1s− SA+) (C and I), per mouse. In vivo σ1s expression in both the hearts and brains of infected animals was evaluated by diaminobenzidine tetrahydrochloride immunohistochemistry with monoclonal antibodies specific for T3 σ1s. Cardiac sections from intramuscularly inoculated mice (T3A, C84, and C84-MA) were stained with monoclonal antibody 2F4 directed against σ1s (A to C) (original magnification, ×200) and polyclonal antisera directed against reovirus structural proteins (D to F) (original magnification, ×100). Coronal sections from intracranially inoculated mice (T3A, C84, and C84-MA) were stained with monoclonal antibody 3E2 directed against σ1s (G to I) (original magnification, ×200) and polyclonal antisera directed against reovirus structural proteins (J to L) (original magnification, ×400). Polyclonal antiserum directed against T3 reovirus structural proteins was used as a control for the presence of reovirus in both cardiac (D to F) and brain (J to L) tissues.