FIG. 5.
Southern blot analysis of cytoplasmic replicative intermediates isolated from PDH after infection with the viral particles from culture fluids of transfected LMH cells. PDH were infected with 1.5 × 107 virus particles purified from the supernatants of transfected LMH cells. Each lane contains one-fourth of the viral DNA recovered from PDH cultures maintained on 60-mm-diameter tissue culture dishes. The positions of the relaxed circle (RC), double-stranded linear (DL), and single-stranded (SS) DNAs are indicated. The marker contains 30 pg of cloned unit-length DHBV DNA migrating at 3 kbp.