TABLE 3.
In situ hybridization detection of viral nucleic acids in tissues collected from infected ducksa
| Virus | Resultc for:
|
|||
|---|---|---|---|---|
| Liverb | Spleen (germinal centers) | Pancreas | Kidney (tubular epithelium) | |
| DHBV (6 × 107) | >95% + | + | − | − |
| MDHBVa | >95% + | − | − | − |
| MDHBVb | >95% + | + | − | ++ |
| CWHBV | >95% + | + | − | − |
| PTHBV | >95% + | + | − | ++ |
| OSHBV | >95% + | + | − | − |
a
In situ hybridization was carried out as described in Materials and Methods and as illustrated in Fig. 7.
b
Infection of both bile ductular epithelium and hepatocytes was detected.
c
+, infected cells detected by in situ hybridization; −, infected cells not detected.