Figure 3.

Phagocytosis and macropinocytosis are not defective in vps13F KO cells. Wild‐type (WT) or knock‐out (KO) cells were incubated for 20 min with fluorescent latex beads, heat‐inactivated Klebsiella pneumoniae, or dextran. The internalized fluorescence was measured by flow cytometry. Mean fluorescence was plotted for each strain and expressed as a function of internalization in WT cells. Macropinocytosis of dextran and phagocytosis of beads or bacteria were as efficient in vps13F KO as in WT cells (mean ± SEM; 5 and 7 independent experiments for WT and KO respectively). Kil2–vps13F KO cells exhibited a minor defect in phagocytosis of latex beads compared to WT cells, as also seen in kil2 KO cells (p < .05)