Figure 6.

Vps13F KO cells are defective for killing Klebsiella pneumoniae but not Bacillus subtilis. (a) WT or KO Dictyostelium were mixed with K. pneumoniae (200 Dictyostelium cells per bacteria to ensure optimal phagocytosis). At the indicated times, an aliquot of the mixture was collected, Dictyostelium cells were lysed, the bacteria were plated on LB‐agar, and the total (extracellular and intracellular) number of remaining viable bacteria was evaluated by counting colony forming units (CFUs). Results are expressed as a percentage of CFUs at time 0 (mean ± SEM; *: p < .01; student t test; n = 12 for WT or 16 for vps13F KO). (b) Intracellular killing of B. subtilis was assessed as described in (a). No significant difference was detected between WT and vps13F KO cells (n = 4)