FIG. 1.

Conserved amino acid regions in pIX. (A) Amino acid sequence alignment of all available primate Ad pIX proteins (CLUSTAL W) (19). Fully conserved residues are shaded black. Residues that occur in more than 50% of the sequences are shaded gray. Lines under the aligned sequences indicate the deletions mentioned in the text. (B) DNA sequence of the human Ad type 5 pIX gene. Deletions of the three conserved domains were introduced by site-directed mutation PCR. The lines depict the DNA sequence deletions. (C) Schematic representation of the pIX variants used for coprecipitation assays in this study. Molecular weights are in thousands.