FIG. 1.

Cell lines latently infected with RRV-GFP. (A) BJAB or 293 cells were infected with RRV-GFP and passaged for several weeks. The BJAB-RRV-GFP-positive cells were sorted and pooled by flow cytometry. Cell lines were passaged in GCV to confirm the persistence of latent infection. (B) RRV can undergo lytic replication in RhFs and HUVEC but not in 293 or BJAB cells. Cells were infected with RRV-GFP at an MOI of ≥1, and infections were carried out for 72 h. Cell-free supernatants were harvested, and viral infectivity was determined by plaque assay.