Table 2.
An RNAi screen to identify the nuclear membrane protein involved in inhibition of CED-3 autoactivation
| Gene | Protein | RNAi phenotype | Cell corpse number | Survival rate |
|---|---|---|---|---|
| npp-1 | Nuclear pore complex protein | − | 0.06 | 37% |
| npp-2 | Nuclear pore complex protein | − | 0 | 4% |
| npp-3 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-4 | Nuclear pore complex protein | − | 0.06 | 6% |
| npp-5 | Nuclear pore complex protein | − | 0.06 | 23% |
| npp-6 | Nuclear pore complex protein | − | 0 | 4% |
| npp-7 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-8 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-9 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-10 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-12 | Nuclear pore complex protein | − | 0.11 | 65% |
| npp-14 | Nuclear pore complex protein | + | 0.73 | 37% |
| npp-15 | Nuclear pore complex protein | − | 0.13 | 13% |
| npp-16 | Nuclear pore complex protein | − | 0.07 | 36% |
| npp-17 | Nuclear pore complex protein | − | 0 | 51% |
| npp-18 | Nuclear pore complex protein | − | 0.07 | 78% |
| npp-19 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-20 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-21 | Nuclear pore complex protein | lethal | ND | 0% |
| npp-23 | Nuclear pore complex protein | − | 0.10 | 78% |
| unc-84 | Inner nuclear membrane protein | − | 0.06 | 71% |
| sun-1 | Inner nuclear membrane protein | − | 0 | 10% |
| unc-83 | Outer nuclear membrane protein | − | 0 | 70% |
| anc-1 | Outer nuclear membrane protein | − | 0.13 | 74% |
| zyg-12 | Outer nuclear membrane protein | − | 0.13 | 2% |
| lem-2 | Lamin-binding protein | − | 0.12 | 73% |
| lem-3 | Lamin-binding protein | − | 0 | 72% |
| emr-1 | Lamin-binding protein | − | 0.06 | 78% |
| ran-1 | Nuclear import/export | lethal | ND | 0% |
| ran-2 | Nuclear import/export | − | 0 | 67% |
| ran-3 | Nuclear import/export | − | 0.07 | 74% |
| ran-4 | Nuclear import/export | − | 0 | 11% |
| ran-5 | Nuclear import/export | − | 0.07 | 61% |
| ima-1 | Importin alpha family | − | 0.18 | 67% |
| ima-2 | Importin alpha family | − | 0 | 13% |
| ima-3 | Importin alpha family | − | 0.07 | 27% |
| imb-2 | Importin beta family | − | 0.07 | 48% |
| imb-6 | Importin beta family | − | 0.13 | 67% |
At least 12 ced-1(e1735); ced-3(n718 G65R) embryos were scored in each RNAi experiment. Animals treated with control RNAi had a mean of 0.07 cell corpses (n=15). “−” indicates RNAi treatment with no detectable effect. “+” indicates RNAi treatment that substantially increased the number of persistent cell corpses. RNAi knockdown of several genes resulted in the “lethal” phenotype with no progeny, which prevented cell corpse counting (not determined or ND).