Skip to main content
. Author manuscript; available in PMC: 2018 Jun 10.
Published in final edited form as: J Control Release. 2017 Mar 31;255:12–26. doi: 10.1016/j.jconrel.2017.03.389

Figure 7.

Figure 7

Leading DB4-PDB12 ternary si-NPs have higher cytosolic delivery than the parent binary si-NPs. (A) DB4-PDB12 si-NPs are similar size (DB4-PDB12: 124.4 vs PDB: 129.5 nm) as PDB si-NPs. (B) DB4-PDB12 have increased cell uptake over the PDB parent si-NPs (60× mag). (C) DB4-PDB12 si-NPs exhibit significantly lower endolysosomal colocalization than PDB or Lipofectamine 2000 (LF2K; administered at the maximum tolerated dose of 25 nM) si-NPs (n ≥ 4 fields of view; 60× mag). (D) Increased DB4-PDB12 cell uptake visualized by confocal microscopy. (E) Increased cytosolic delivery of cargo by DB4-PDB12 si-NPs visualized by confocal microscopy in cells with acidic endolysosomal vesicles co-stained with Lysotracker Red (75 nM).