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Schematic representation of ADS-MVA construction (top and right panels) and the characterization of ADS-MVA by using avian cells (left bottom panel). The SARS-S gene was introduced, together with GFP gene, each under a separate promoter, into the Del III region of the MVA genome. (A) S glycoprotein was detected on CEF cells by using an anti-S polyclonal antibody (WH) in an immunofluorescence assay. (B) GFP coexpressed on the same cell population as seen in panel A.
