FIG. 5.

Activation of mouse macrophage cell line by AcNPV DNA. (A) Methylation status of genomic DNA. Genomic DNAs obtained from AcNPV, Sf-9 cells, E. coli, and 293T cells were digested with the methylation-sensitive restriction enzyme HpaII. Undigested (−) and digested (+) samples were analyzed by agarose gel electrophoresis. (B) RAW264.7 cells (10⁶ cells/well) were treated with AcNPV DNA (5 μg/ml) or PGN (2.5 μg/ml) in the absence (−) or presence (+) of liposomes for 24 h, and the production of TNF-α in culture supernatants was determined by a sandwich ELISA. Data are shown as means ± SD. (C) Activation of RAW264.7 cells (10⁶ cells/well) inoculated with untreated or UV-inactivated AcNPV (5 μg/ml) in the presence or absence of liposomes was assessed by the production of TNF-α in culture supernatants. Data are shown as means ± SD.