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Expression of cytokine mRNAs in the NALTs. Total RNA was extracted from the NALTs of mice intranasally treated with 1 μg of PR8 vaccine with or without poly(I:C). The mRNA levels of IFN-α (A), IFN-β (B), IFN-γ (C), IL-4 (D), IL-6 (E), and IL-12 p40 (F) in the NALTs were determined with real time RT-PCR (n = 3). *, P < 0.05 versus the pretreated group (0 h).
